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src kinase-specific inhibitor (pp1)  (AG Scientific)

 
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    AG Scientific src kinase-specific inhibitor (pp1)
    Src Kinase Specific Inhibitor (Pp1), supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/src kinase-specific inhibitor (pp1)/product/AG Scientific
    Average 90 stars, based on 1 article reviews
    src kinase-specific inhibitor (pp1) - by Bioz Stars, 2026-03
    90/100 stars

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    Inhibition of protein tyrosine kinase activity blocks TCR down-regulation. P14 TCR transgenic spleen cells (A, B) or P14.G5 CTL clone (C, D) were preincubated for 60 min in the presence of <t>PP1</t> or genistein. Preincubation with herbimycin A was done overnight with P14 spleen cells or for 60 min with P14.G5 CTL. EL-4 cells pulsed with p33 (1 μM) were then added for 4 h and cells stained for flow cytometry with anti-CD8 and anti-Vα2 antibodies (A, C) or with anti-CD8 and anti-CD69 antibodies (B, D). Mean fluorescence intensities are expressed as % or MFI of control in the absence of peptide.
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    Inhibition of protein tyrosine kinase activity blocks TCR down-regulation. P14 TCR transgenic spleen cells (A, B) or P14.G5 CTL clone (C, D) were preincubated for 60 min in the presence of PP1 or genistein. Preincubation with herbimycin A was done overnight with P14 spleen cells or for 60 min with P14.G5 CTL. EL-4 cells pulsed with p33 (1 μM) were then added for 4 h and cells stained for flow cytometry with anti-CD8 and anti-Vα2 antibodies (A, C) or with anti-CD8 and anti-CD69 antibodies (B, D). Mean fluorescence intensities are expressed as % or MFI of control in the absence of peptide.

    Journal:

    Article Title: Transient alteration of T cell fine specificity by a strong primary stimulus correlates with T cell receptor down-regulation

    doi:

    Figure Lengend Snippet: Inhibition of protein tyrosine kinase activity blocks TCR down-regulation. P14 TCR transgenic spleen cells (A, B) or P14.G5 CTL clone (C, D) were preincubated for 60 min in the presence of PP1 or genistein. Preincubation with herbimycin A was done overnight with P14 spleen cells or for 60 min with P14.G5 CTL. EL-4 cells pulsed with p33 (1 μM) were then added for 4 h and cells stained for flow cytometry with anti-CD8 and anti-Vα2 antibodies (A, C) or with anti-CD8 and anti-CD69 antibodies (B, D). Mean fluorescence intensities are expressed as % or MFI of control in the absence of peptide.

    Article Snippet: 4.13 Inhibition of src family kinase activity P14.G5 CTL (6×10 4 /well) or P14 spleen cells (5×10 5 /well) were preincubated for 60 min with the src family kinase-specific inhibitor PP1 (10 μM and 50 μM, respectively) (Bio-mol, Plymouth Meeting, PA) [ 28 ].

    Techniques: Inhibition, Activity Assay, Transgenic Assay, Staining, Flow Cytometry, Fluorescence, Control